Part:BBa_K5194007

pET 28a - 20061 plasmid vector

The E. coli expression vector has Kanamycin resistance, with 5' NcoI and 3' HindIII cloning sites. The plasmid has lac operator for controlled induction with IPTG, His-tag on N-terminus followed by spacer sequence and TEV protease site.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5204
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NotI site found at 7
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 5138
  Illegal XhoI site found at 16
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5204
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5204
  Illegal NgoMIV site found at 315
  Illegal NgoMIV site found at 3358
  Illegal NgoMIV site found at 3518
  Illegal NgoMIV site found at 5106
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 2440